Document 0037
 DOCN  M9490037
 TI    Major expansion of CD8+ T cells with a predominant V beta usage during
       the primary immune response to HIV [see comments]
 DT    9411
 AU    Pantaleo G; Demarest JF; Soudeyns H; Graziosi C; Denis F; Adelsberger
       JW; Borrow P; Saag MS; Shaw GM; Sekaly RP; et al; Laboratory of
       Immunoregulation, National Institute of Allergy and; Infectious
       Diseases, National Institutes of Health, Bethesda,; Maryland 20892.
 SO    Nature. 1994 Aug 11;370(6489):463-7. Unique Identifier : AIDSLINE
       MED/94322938
 CM    Comment in: Nature 1994 Aug 11;370(6489):416
 AB    A SIGNIFICANT proportion (up to 70%) of individuals experience an acute
       clinical syndrome of varying severity associated with primary infection
       with the human immunodeficiency virus (HIV). We report here studies on
       six individuals who showed an acute HIV syndrome which generally
       resolved within four weeks, concomitant with a dramatic downregulation
       of viraemia. To characterize the T-cell-mediated primary immune response
       to HIV, we used combined semiquantitative polymerase chain reaction
       assay and cytofluorometry to analyse the T-cell antigen receptor
       repertoire in sequential peripheral blood mononuclear cells from the
       patients. We found major oligoclonal expansions in a restricted set of
       variable-domain beta-chain (V beta) families. Cells expressing the
       expanded V beta s predominantly expressed the CD8 T-cell differentiation
       antigen and mediated HIV-specific cytotoxicity. Major oligoclonal
       expansions of these CD8+ T lymphocytes may represent an important
       component of the primary immune response to viral infections and may
       help to clarify both the immunopathogenic and the protective mechanisms
       of HIV infection.
 DE    Adolescence  Adult  Amino Acid Sequence  *Antigens, CD8  Cell Line,
       Transformed  Cells, Cultured  Clone Cells  Cytotoxicity, Immunologic
       DNA  Female  Flow Cytometry  Human  HIV/*IMMUNOLOGY  HIV
       Infections/*IMMUNOLOGY  Immunogenetics  Immunophenotyping  Male
       Molecular Sequence Data  Polymerase Chain Reaction  Prospective Studies
       Receptors, Antigen, T-Cell, alpha-beta/*GENETICS  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  T-Lymphocyte Subsets/*IMMUNOLOGY
       JOURNAL ARTICLE

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